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Postdoctoral Scientist - Melmed Lab - Department of Medicine

Company: Cedars-Sinai
Location: Alhambra
Posted on: May 24, 2023

Job Description:

Are you looking to contribute to groundbreaking research? We look forward to having you join our team!Cedars-Sinai is an international leader in biomedical research, we translate discoveries into successful treatments with global impact.Dr. Shlomo Melmed, MD is looking for a Postdoctoral Scientist to join a diverse team!The Melmed Laboratory focuses on elucidating mechanisms of pituitary tumor development and pathogenesis, translating the study of anterior pituitary function regulation to the clinic and establishing new modalities of diagnosis and treatment for pituitary diseases.Working independently but in close cooperation and in consultation with the Principal Investigator and other Research Scientists, the Postdoctoral Scientist will perform routine and complex laboratory procedures throughout training period. The incumbent may develop, adapt, and implement new research techniques and protocols. This position is not responsible for generating grant funds.Primary Duties and Responsibilities:

  • May assist in preparation of grant proposals.
  • May participate in publications and presentations as author or co-author.
  • Designs and performs experiments.
  • Will keep appropriate experimental records and documentation, and analyze the results.
  • Analyzes, interprets, summarizes, and compiles data.
  • Operates and maintains equipment and instruments.
  • Will investigate the mechanisms of pituitary tumorigenesis.Department-Specific Responsibilities:Specific laboratory procedures conducted:
  • Plasmid construction
  • DNA/RNA/protein extraction
  • SDS-polyacrylamide gel electrophoresis
  • Western blotting
  • Immunoprecipitation
  • Luciferase reporter gene assay
  • Site-directed mutagenesis
  • Glutathione S-transferase (GST) pull-down experiments
  • PCR, RT-PCR, quantitative real-time PCR, ELISA, IgG purification
  • DNA digestion, DNA amplification, ligation and sub-cloning, DNA or siRNA transfection, and direct sequencing
  • Mammalian cell culture and transfection
  • Blood and organ collection (from rats and mice)
  • Isolation of primary hepatocyte (rat)
  • Primary culture of anterior pituitary cells (mice)
  • Injection into tail vein of mice
  • In vivo knockdown experiment by antisense oligonucleotide--Education:Doctorate (MD, PhD, VMD, or DDS) in area directly related to field of research specialization is required.Experience:
    • Acquires technical and theoretical knowledge of research project and objectives during one to five (1-5) year post-doctoral appointment.
    • Works independently on research projects designed by a mentor (typically the PI) within area of specialization.
    • Demonstrated ability to perform experimental protocols and procedures, including detailed data collection, and analysis and operation and maintenance of specialized equipment.Physical Demands: Able to perform moderate lifting. Able to sit, stand and walk for prolonged periods of time. Able to read papers and online documents. Able to operate standard office equipment. Able to exercise physical ability and perspective acuity to satisfactorily perform essential job functions.Working Title: Postdoctoral Scientist - Melmed Lab - Department of MedicineDepartment: Home Dept - Melmed LabBusiness Entity: Cedars-Sinai Medical CenterJob Category: Academic/ResearchJob Specialty: PostdoctoralPosition Type: Full-timeShift Length: 8 hour shiftShift Type: DayBase Pay:$64,500.00 - $93,600.00

Keywords: Cedars-Sinai, Alhambra , Postdoctoral Scientist - Melmed Lab - Department of Medicine, Healthcare , Alhambra, California

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